RFLP

 Restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences, known as polymorphisms, in order to distinguish individuals, populations, or species or to pinpoint the locations of genes within a sequence.

 In RFLP analysis, a DNA sample is digested into fragments by one or more restriction enzymes, and the resulting restriction fragments are then separated by gel electrophoresis according to their size and transferred to a membrane via the Southern blot procedure. Hybridization of the membrane to a labelled DNA probe then determines the length of the fragments which are complementary to the probe.

A restriction fragment length polymorphism is said to occur when the length of a detected fragment varies between individuals, indicating non-identical sequence homologies.

RFLP analysis was an important early tool in genome mapping, localization of genes for genetic disorders, determination of risk for disease, and paternity testing. Most of the researchers use RFLP method to detect polymorphisms in various disorders.

Journal of Clinical Genomics publishes articles related to the RFLP analysis and is inviting researchers to associate and publish with the journal.

Regards,

Editorial Assistant,

Journal of Clinical Genomics,

Email: genomics@peerjournal.org