Journal of Blood Research & Hematologic Diseases is an open access journal and celebrating the 5^th Anniversary, an esteemed journal which mainly deals with Blood Research & Hematologic Diseases.

This Journal of Blood Research & Hematologic Diseases is a peer reviewing processing journal and publishes the articles on Blood Vessels, Circulatory System, Haematology, Haematopoiesis, Haemoglobin, Haemostasis

The current study evaluates the anticoagulant and antiplatelet activities of Pisum sativum pod extract (PSPE). PSPE revealed similar protein banding pattern under both reducing and nonreducing conditions on SDS-PAGE, suggesting the presence of monomeric proteins in the extract. PSPE showed proteolytic activity as it hydrolyzed casein and gelatin and the specific activity was found to be 0.16 and 0.21 units/mg/min respectively. The proteolytic activity of PSPE was completely abolished by PMSF but not IAA, EDTA and 1,10, Phenanthroline suggesting the presence of serine protease. Further, PSPE was evaluated for anticoagulation efficiency. Interestingly, PSPE increased the clotting time of citrated human plasma from the control 220 sec to 460 sec suggesting its anticoagulant property.

Furthermore, PSPE specifically prolonged the clotting time of APTT but not PT revealed the interference in intrinsic pathway of coagulation cascade. In addition, PSPE exhibited anti-aggregation activity by inhibiting agonists such as ADP and epinephrine induced platelet aggregation in a dose dependent manner and the detected aggregation inhibition was found to be 58% and 82% respectively. In addition, PSPE preferentially hydrolyzed Aα and Bβ chain of human fibrinogen and all the chains of fibrin clot. Fibrinogenolytic and fibrinolytic activity of PSPE was inhibited by PMSF but not IAA, EDTA and 1,10, Phenanthroline suggesting the presence of serine protease in the extract. Moreover, PSPE did not hydrolyze RBC cells signifying its non-toxic property.

 Journal of Blood Research & Hematologic Diseases is using Editorial Tracking System for quality in the review process. Editorial Tracking is an online manuscript submission, review and tracking systems used by most of the best open access journals. Review processing is performed by the editorial board members and Diagnosis or outside experts; at least two independent reviewers approval followed by editor's approval is required for acceptance of any citable manuscript. Kindly submit your article online or Email: bloodsci@emedsci.com  (or) bloodres@peerjournal.org

Media contact:
Eliza Grace
Journal of Surgical Pathology and Diagnosis
Email : bloodsci@emedsci.com

Journal of Blood Research & Hematologic Diseases is an open access journal and celebrating the 5^th Anniversary, an esteemed journal which mainly deals with Blood Research & Hematologic Diseases.

This Journal of Blood Research & Hematologic Diseases is a peer reviewing processing journal and publishes the articles on Blood Vessels, Circulatory System, Haematology, Haematopoiesis, Haemoglobin, Haemostasis

The current study evaluates the anticoagulant and antiplatelet activities of Pisum sativum pod extract (PSPE). PSPE revealed similar protein banding pattern under both reducing and nonreducing conditions on SDS-PAGE, suggesting the presence of monomeric proteins in the extract. PSPE showed proteolytic activity as it hydrolyzed casein and gelatin and the specific activity was found to be 0.16 and 0.21 units/mg/min respectively. The proteolytic activity of PSPE was completely abolished by PMSF but not IAA, EDTA and 1,10, Phenanthroline suggesting the presence of serine protease. Further, PSPE was evaluated for anticoagulation efficiency. Interestingly, PSPE increased the clotting time of citrated human plasma from the control 220 sec to 460 sec suggesting its anticoagulant property.

Furthermore, PSPE specifically prolonged the clotting time of APTT but not PT revealed the interference in intrinsic pathway of coagulation cascade. In addition, PSPE exhibited anti-aggregation activity by inhibiting agonists such as ADP and epinephrine induced platelet aggregation in a dose dependent manner and the detected aggregation inhibition was found to be 58% and 82% respectively. In addition, PSPE preferentially hydrolyzed Aα and Bβ chain of human fibrinogen and all the chains of fibrin clot. Fibrinogenolytic and fibrinolytic activity of PSPE was inhibited by PMSF but not IAA, EDTA and 1,10, Phenanthroline suggesting the presence of serine protease in the extract. Moreover, PSPE did not hydrolyze RBC cells signifying its non-toxic property.

 Journal of Blood Research & Hematologic Diseases is using Editorial Tracking System for quality in the review process. Editorial Tracking is an online manuscript submission, review and tracking systems used by most of the best open access journals. Review processing is performed by the editorial board members and Diagnosis or outside experts; at least two independent reviewers approval followed by editor's approval is required for acceptance of any citable manuscript. Kindly submit your article online or Email: bloodsci@emedsci.com  (or) bloodres@peerjournal.org

Media contact:
Eliza Grace
Journal of Surgical Pathology and Diagnosis
Email : bloodsci@emedsci.com